Virulence and Reproduction of Entomopathogenic Nematodes Isolated from a Single Mexican Locality1

Entomopathogenic nematodes (EPNs) are important biological control agents of insect pests. Strains or isolates obtained in specific regions and utilized situ might contribute to the success EPNs, since they adapted local abiotic conditions. We isolated identified three each Heterorhabditis bacteriophora Poinar Steinernema carpocapsae (Weiser) from soils at Saltillo, Coahuila State, Mexico. These six were bioassayed against larvae Tenebrio molitor L., using concentrations infective juveniles (IJs) per larva (0, 10, 25, 50, 100, 200). EPN-induced mortality ranged 15.2% 100%. The designated M5 isolate S. caused 100% after 72 h ≥25 IJs/larva with a median lethal concentration 4.99 IJs/larva. Comparison levels induced same EPN indicated that isolates, general, higher than isolates. IJ production T. also was greatest following exposure 100 mean 17,320 Based on production, appears be viable candidate for further study possible development use pest management programs. This indicates naturally coexisting nematode possess different attributes critical their as biocontrol agents.